Sihlinzeka ngekhithi Yokuhlola Izidakamizwa okuwukuhlola okusheshayo, okuhlola ukutholwa kwekhwalithi yesidakamizwa esisodwa/eziningi kanye nama-metabolite ezidakamizwa emchameni womuntu ngamaleveli anqanyuliwe athile. Isetshenziselwa uchwepheshe kuphela futhi isetshenziselwa ukuxilonga i-in vitro kuphela.
Funda kabanziThumela UphenyoIkhithi Yokuhlola Izidakamizwa Ezisheshayo Esiyisinyathelo Esiyinye iwusizo kakhulu.Amathe ayingxube eyinkimbinkimbi engagcini nje ngokuqukethe amaprotheni ahlukahlukene, kodwa futhi i-DNA, i-RNA, ama-fatty acids nama-microorganisms ahlukahlukene. Ucwaningo luthole ukuthi izingxenye zamaprotheni ezinhlobonhlobo ezisegazini zikhona ematheni, ezingakhombisa izinguquko emazingeni amaprotheni ahlukahlukene egazini. Ngakho-ke, kungenzeka ukuxilonga lesi sifo ngokuhlolwa kwamathe.
Funda kabanziThumela UphenyoIsinyathelo esisodwa esisheshayo Ukuhlolwa kwezidakamizwa eziningi kwamathe 5 kuphaneli yokuhlolwa kwezidakamizwa oku-1: Amathe ayingxube eyinkimbinkimbi equkethe hhayi kuphela amaprotheni ahlukahlukene, kodwa futhi i-DNA, i-RNA, ama-fatty acids nama-microorganisms ahlukahlukene. Lolu cwaningo luthole ukuthi izingxenye zamaprotheni ezinhlobonhlobo ezisegazini zikhona ematheni, ezingakhombisa izinguquko emazingeni amaprotheni ahlukahlukene egazini. Ngakho-ke, kungenzeka ukuxilonga lesi sifo ngokuhlolwa kwamathe.
Funda kabanziThumela UphenyoUmchamo ovunyiwe we-CE Isidakamizwa se-DOA inkomishi yokuhlola okusheshayo: Ukuhlolwa komchamo ukuhlolwa kwezokwelapha. Kubandakanya ukuhlaziywa komchamo okujwayelekile, ukutholwa kwezingxenye ezibonakalayo zomchamo (ezifana namangqamuzana egazi abomvu omchamo, amangqamuzana amhlophe egazi, njll.), izingxenye zamaprotheni ukuzimisela kobuningi, ukuzimisela kwe-enzyme yomchamo, njll. Ukuhlolwa komchamo kubaluleke kakhulu ekuxilongweni komtholampilo, umphumela wokwelapha kanye nokubikezela.
Funda kabanziThumela UphenyoIzidakamizwa eziningi 3 kuphaneli yokuhlola ye-1 Imithi yokuhlola Ukuhlukunyezwa: Ukuhlolwa komchamo kuwukuhlolwa kwezokwelapha. Kubandakanya ukuhlaziywa komchamo okujwayelekile, ukutholwa kwezingxenye ezibonakalayo zomchamo (ezifana namangqamuzana egazi abomvu omchamo, amangqamuzana amhlophe egazi, njll.), izingxenye zamaprotheni ukuzimisela kobuningi, ukuzimisela kwe-enzyme yomchamo, njll. Ukuhlolwa komchamo kubaluleke kakhulu ekuxilongweni komtholampilo, umphumela wokwelapha kanye nokubikezela.
Funda kabanziThumela Uphenyo12 ipharamitha test reagent umchamo: Ukuhlolwa umchamo ukuhlolwa kwezokwelapha. Kubandakanya ukuhlaziywa komchamo okujwayelekile, ukutholwa kwezingxenye ezibonakalayo zomchamo (ezifana namangqamuzana egazi abomvu omchamo, amangqamuzana egazi amhlophe, njll.), ukuzimisela kwezingxenye zamaprotheni, ukunqunywa kwe-enzyme yomchamo, njll. Ukuhlolwa komchamo kubaluleke kakhulu ekuxilongweni komtholampilo, umphumela wokwelapha kanye nokubikezela.
Funda kabanziThumela Uphenyo